eLife6d
Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
By use of the lens system and CMOS cameras, a wide-field epi-illumination fluorescence imaging system was constructed. The imaging system chassis was designed to accommodate both inverted and upright ...
Frontiers6d
Group ICA of wide-field calcium imaging data reveals the retrosplenial cortex as a major contributor to cortical activity during anesthesia
Wide-field imaging was performed in a dark room using a custom-made microscope. The excitation source for jRCaMP1b ... United States). The fluorescence signal of jRCaMP1b was collected through a ...
Frontiers6d
Raw Data to Results: A Hands-On Introduction and Overview of Computational Analysis for Single-Molecule Localization Microscopy
Single-molecule localization microscopy (SMLM) is an advanced microscopy method that uses the blinking of fluorescent molecules to determine the position of these molecules with a resolution below the ...