Large-scale microscopy combined with tissue clearing and expansion enables nanoscale imaging of centimeter scale specimens.

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Super-resolution microscopy includes a variety of microscopy techniques that increase the resolving ability of a light microscope well beyond the classical limits dictated by the diffraction barrier.

For Ca 2+ imaging experiments, we used a Yokogawa CSU-X1A spinning-disk confocal scanner, mounted on an upright microscope (Zeiss Examiner) with a 63X, 1.0 NA objective (W Plan-Apochromat, Zeiss).